H00003815-M03

antibody from Abnova Corporation

Targeting: KIT C-Kit, CD117, PBT, SCFR

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003815-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003815-M03, RRID:AB_509117

Product name

KIT monoclonal antibody (M03), clone 3A8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant KIT.

Antigen sequence

PGKSDLIVRVGDEIRLLCTDPGFVKWTFEILDETN
ENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVF
VRDPAKLFLVDRSLYGKEDNDTLVRCPLTD


Isotype

IgG

Antibody clone number

3A8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of KIT expression in transfected 293T cell line by KIT monoclonal antibody (M03), clone 3A8.Lane 1: KIT transfected lysate(109.865 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged KIT is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol