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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 323416 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#323416, RRID:AB_2131441
- Product name
- PerCP/Cy5.5 anti-human CD117 (c-kit)
- Antibody type
- Monoclonal
- Antigen
- MOLM-1 megakaryocitic cell line
- Description
- CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemic cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117, and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells. The A3C6E2 antibody potently blocks the binding of SCF.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Far-Red dye
- Isotype
- IgG
- Vial size
- 100 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human erythroleukemia cell line, HEL, was stained with CD117 (c-kit) (clone A3C6E2) PerCP/Cy5.5 (filled histogram) or mouse IgG1, κ PerCP/Cy5.5 isotype control (open histogram).
