HPA004471

antibody from Atlas Antibodies

Targeting: KIT C-Kit, CD117, PBT, SCFR

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA004471

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA004471, RRID:AB_1078431

Product name

Anti-KIT

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VGDEIRLLCTDPGFVKWTFEILDETNENKQNEWIT
EKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFL
VDRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGK
PLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVD
Q


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human breast and prostate tissues using Anti-KIT antibody. Corresponding KIT RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human breast shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows low expression as expected.

Sample type

HUMAN