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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
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- Product number
- GTX104477 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104477, RRID:AB_1949729
- Product name
- BCKDK antibody [N2C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse brain 10% SDS PAGE GTX104477 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:HeLa S3(GTX14654)B:Raji(GTX27908)10% SDS PAGEGTX104477 diluted at 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCF-7 whole cell and membrane extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCKDK(GTX104477) antibody at 1:100 dilution.
