ABIN359244

antibody from antibodies-online

Targeting: BCKDK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN359244

Provider

antibodies-online

Product name

anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (Middle Region) antibody

Antibody type

Polyclonal

Antigen

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human BCKDK.

Description

Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Reactivity

Human

Host

Rabbit

Epitope

Middle Region

Vial size

0.4 mL

Concentration

0.25 mg/mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Solution structure and dynamics of the lipoic acid-bearing domain of human mitochondrial branched-chain alpha-keto acid dehydrogenase complex.

Chang CF, Chou HT, Chuang JL, Chuang DT, Huang TH
The Journal of biological chemistry 2002 May 3;277(18):15865-73

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Branched-chain alpha-ketoacid dehydrogenase kinase. Molecular cloning, expression, and sequence similarity with histidine protein kinases.

Popov KM, Zhao Y, Shimomura Y, Kuntz MJ, Harris RA
The Journal of biological chemistry 1992 Jul 5;267(19):13127-30

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.