Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-79127 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP24A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Vitamin D(3) Metabolic Enzymes in the Porcine Uterus: Expression, Localization and Autoregulation by 1,25(OH)(2)D(3) In Vitro.
Grzesiak M, Kaminska K, Bodzioch A, Drzewiecka EM, Franczak A, Knapczyk-Stwora K
International journal of molecular sciences 2022 Apr 2;23(7)
International journal of molecular sciences 2022 Apr 2;23(7)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP24A1 in Lane 1: rat thymus tissue lysate, Lane 2: rat lung tissue lysate, Lane 3: HeLa whole cell lysate, Lane 4: 22RV1 whole cell lysate, Lane 5: A431 whole cell lysate, Lane 6: HEPA whole cell lysate using 40-50 µg per well. Sample was incubated with CYP24A1 (Product # PA5-79127) at a dilution of 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP24A1 on paraffin-embedded mouse kidney tissue. Sample was incubated with CYP24A1 polyclonal antibody (Product# PA5-79127).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP24A1 on paraffin-embedded human Pancreatic cancer tissue. Sample was incubated with CYP24A1 polyclonal antibody (Product# PA5-79127).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP24A1 on paraffin-embedded rat kidney tissue. Sample was incubated with CYP24A1 polyclonal antibody (Product# PA5-79127).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Representative micrographs of CYP24A1 immunofluorescent localization within porcine uterus on days 2-5 ( a ), 10-12 ( b ), 15-16 ( c ) and 18-20 ( d ) of the estrous cycle. Immunoreactive proteins were visualized using a Cy3 detection system (red). Nuclei were counterstained with DAPI (blue). Positive signal ( arrows ) was only found in the cytoplasm of luminal (LE) and glandular (GE) epithelial cells of the endometrium. Negative control ( d inset) was obtained by the replacement of primary antibody by non-immune rabbit IgG. S, stroma; asterisks (*), blood vessels. Bar = 50 um.