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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 21582-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP24A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Aliquoting is unnecessary for -20°C storage.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 400 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 21582-1-AP (CYP24A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of CYP24A1 antibody (21582-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP24A1 transfected A549 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP Result of anti-CYP24A1 (IP:21582-1-AP, 4 µg; Detection:21582-1-AP 1:300) with HeLa cells lysate 2800 µg.
