Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21704 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP24A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.46 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP24A1 using 30µg of A) 293T (B) A431 (C) H1299 (D) HeLa S3 (E) HepG2 (F) MOLT4 and G) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CYP24A1 polyclonal antibody (Product # PA5-21704) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CYP24A1 Polyclonal Antibody (Product # PA5-21704). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP24A1 Polyclonal Antibody (Product # PA5-21704) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts of Mouse Kidney (Lane 1), Mouse Bladder (Lane 2), Mouse Lung (Lane 3), Mouse Liver (Lane 4), Mouse Skeletal Muscle (Lane 5) and Mouse Adipose (Lane 6). The blot was probed with Anti-CYP24A1 Polyclonal Antibody (Product # PA5-21704, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 59 kDa band corresponding to CYP24A1 was detected across tissues tested and the protein levels were found to be low in Mouse Skeletal Muscle when compared to other tissues.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CYP24A1 Polyclonal Antibody detects CYP24A1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CYP24A1 Polyclonal Antibody (Product # PA5-21704) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.