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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2944 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2944, RRID:AB_1578066
- Product name
- NLK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NLK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling.
Regulation of lymphoid enhancer factor 1/T-cell factor by mitogen-activated protein kinase-related Nemo-like kinase-dependent phosphorylation in Wnt/beta-catenin signaling.
Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells.
Ishitani T, Kishida S, Hyodo-Miura J, Ueno N, Yasuda J, Waterman M, Shibuya H, Moon RT, Ninomiya-Tsuji J, Matsumoto K
Molecular and cellular biology 2003 Jan;23(1):131-9
Molecular and cellular biology 2003 Jan;23(1):131-9
Regulation of lymphoid enhancer factor 1/T-cell factor by mitogen-activated protein kinase-related Nemo-like kinase-dependent phosphorylation in Wnt/beta-catenin signaling.
Ishitani T, Ninomiya-Tsuji J, Matsumoto K
Molecular and cellular biology 2003 Feb;23(4):1379-89
Molecular and cellular biology 2003 Feb;23(4):1379-89
Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells.
Yasuda J, Tsuchiya A, Yamada T, Sakamoto M, Sekiya T, Hirohashi S
Biochemical and biophysical research communications 2003 Aug 22;308(2):227-33
Biochemical and biophysical research communications 2003 Aug 22;308(2):227-33
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NLK polyclonal antibody (Cat # PAB2944) in A-375 cell lysate. NLK (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NLK polyclonal antibody (Cat # PAB2944) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
