AMAb90841

antibody from Atlas Antibodies

Targeting: BRD4 CAP, HUNK1, HUNKI, MCAP

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb90841

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb90841, RRID:AB_2665685

Product name

Anti-BRD4

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

PQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSP
LMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAAS
VVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPK
HPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAP
PP


Epitope

Binds to an epitope located within the peptide sequence PQPAKPQQVIQHHHS as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL1115

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRD4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-BRD4 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of MCF-7 cells using the Anti-BRD4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach cancer shows strong nuclear positivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human breast cancer shows moderate nuclear immunoreactivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colorectal cancer shows moderate nuclear positivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine shows strong positivity in glandular cells and some lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate nuclear immunoreactivity in a subet of cells in seminiferous tubules.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong nuclear immunoreactivity in renal tubuli and glomeruli.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach cancer shows moderate nuclear positivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows moderate nuclear positivity in cells in renal glomeruli and in tubules.