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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AP21640PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21640PU-N, RRID:AB_10758324
- Product name
- anti BRD4
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 150-200 of human Brd4
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0,5 mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot analysis of Brd4 in human HeLa lysate in the 1) absence and 2) presence of immunizing peptide and C) mouse NIH 3T3 cell lysate using AP21640PU-N at 3 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
