M00123

antibody from Boster Biological Technology

Targeting: BRD4 CAP, HUNK1, HUNKI, MCAP

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00123

Provider

Boster Biological Technology

Product name

Anti-Brd4 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for BRD4 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat BRD4 information: Molecular Weight: 152219 MW; Subcellular Localization: Nucleus. Chromosome. Associates with acetylated chromatin. Released from chromatin upon deacetylation of histones that can be triggered by different signals such as activation of the JNK pathway or nocodazole treatment; Tissue Specificity: Ubiquitously expressed.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

DBH-2

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human kidney, using Brd4 Antibody(M00123)BRD4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BRD4 Antibody (M00123)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image