NBP2-76393

antibody from Novus Biologicals

Targeting: BRD4 CAP, HUNK1, HUNKI, MCAP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-76393

Provider

Novus Biologicals

Product name

Rabbit Monoclonal BRD4 Antibody

Antibody type

Monoclonal

Description

Antigen Affinity-purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 2 - 8 C 1 year from date of receipt

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 by WB of HeLa, HEK293T, Jurkat, MDA-MB-231, Hep-G2, A-549, SW620, and SK-MEL-28 (SK) lysate. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393). Secondary: HRP-conjugated goat anti-rabbit IgG.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of mouse BRD4 by WB of BW5147.3 lysate. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 by WB of HeLa, HEK293T, Jurkat, MDA-MB-231, A-549, SW620, and SK-MEL-28 lysate. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-eEF2.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 in FFPE Hela cells by ICC. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: BRD4 Antibody (BL-149-2H5) or isotype control (unshaded). Secondary: DyLight(R) 650-conjugated goat anti-rabbit IgG.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation Sequencing: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] NBP2-76393.