H00090410-M02

antibody from Abnova Corporation

Targeting: IFT20

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00090410-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00090410-M02, RRID:AB_10616064

Product name

IFT20 monoclonal antibody (M02), clone 3F3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant IFT20.

Antigen sequence

MAKDILGEAGLHFDELNKLRVLDPEVTQQTIELKE
ECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKM
KSLAVSPRLECTGAISAHCKLCLSDSSDSPTSPSR
VGGTTGHRCSELAQIYSKAERSSTAATSSPNSRKE
NAARKVSG


Isotype

IgG

Antibody clone number

3F3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of IFT20 expression in transfected 293T cell line by IFT20 monoclonal antibody (M02), clone 3F3.Lane 1: IFT20 transfected lysate(16 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged IFT20 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to IFT20 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol