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- Western blot [3]
- ELISA [1]
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- Product number
- H00009146-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009146-M01, RRID:AB_539535
- Product name
- Mouse Monoclonal HGS Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HGS - hepatocyte growth factor-regulated tyrosine kinase substrate
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.
The class III kinase Vps34 promotes T lymphocyte survival through regulating IL-7Rα surface expression.
Shao X, Liu Y, Yu Q, Ding Z, Qian W, Zhang L, Zhang J, Jiang N, Gui L, Xu Z, Hong Y, Ma Y, Wei Y, Liu X, Jiang C, Zhu M, Li H, Li H
Cell research 2016 May;26(5):593-612
Cell research 2016 May;26(5):593-612
The class III kinase Vps34 promotes T lymphocyte survival through regulating IL-7Rα surface expression.
McLeod IX, Zhou X, Li QJ, Wang F, He YW
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5051-61
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5051-61
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HGS Antibody (6D11) [H00009146-M01] - Analysis of HGS over-expressed 293 cell line, cotransfected with HGS Validated Chimera RNAi ( Cat # H00009146-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HGS monoclonal antibody (M01), clone 6D11 (Cat # H00009146-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HGS Antibody (6D11) [H00009146-M01] - Analysis of HGS expression in transfected 293T cell line by HGS monoclonal antibody (M01), clone 6D11.Lane 1: HGS transfected lysate(86.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HGS Antibody (6D11) [H00009146-M01] - HGS monoclonal antibody (M01), clone 6D11 Analysis of HGS expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: HGS Antibody (6D11) [H00009146-M01] - Detection limit for recombinant GST tagged HGS is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HGS Antibody (6D11) [H00009146-M01] - Analysis of monoclonal antibody to HGS on HeLa cell. Antibody concentration 10 ug/ml.
