PAB4227

antibody from Abnova Corporation

Targeting: HGS Hrs, Vps27, ZFYVE8

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB4227

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB4227, RRID:AB_1575947

Product name

HGS polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of HGS.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

GRIF1 binds Hrs and is a new regulator of endosomal trafficking.

Kirk E, Chin LS, Li L
Journal of cell science 2006 Nov 15;119(Pt 22):4689-701

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Involvement of Hgs/Hrs in signaling for cytokine-mediated c-fos induction through interaction with TAK1 and Pak1.

Sasaki Y, Sugamura K
The Journal of biological chemistry 2001 Aug 10;276(32):29943-52

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Hgs (Hrs), a FYVE domain protein, is involved in Smad signaling through cooperation with SARA.

Miura S, Takeshita T, Asao H, Kimura Y, Murata K, Sasaki Y, Hanai JI, Beppu H, Tsukazaki T, Wrana JL, Miyazono K, Sugamura K
Molecular and cellular biology 2000 Dec;20(24):9346-55

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The HGS polyclonal antibody (Cat # PAB4227) is used in Western blot to detect HGS in HL-60 cell lysate .

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with HGS polyclonal antibody (Cat # PAB4227) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)