Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF7037 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal SARM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human SARM1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation of Human SARM1. SARM1 was immunoprecipitated from 400 µg of SH-SY5Y human neuroblastoma cell line lysates using 4 µg of Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). SH-SY5Y lysates or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated SARM1 was detected by Western blot under reducing conditions using 1.0 µg/mL Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.
