691402

antibody from BioLegend

Targeting: DAXX DAP6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

691402

Provider

BioLegend

Proper citation

BioLegend Cat#691402, RRID:AB_2632649

Product name

Purified anti-Daxx

Antibody type

Monoclonal

Description

Daxx is a multifunctional protein that interacts with a wide variety of proteins. It was originally identified as a signaling protein that binds to the Fas death domain, enhances Fas-mediated apoptosis and activates the JNK pathway. Later, Daxx was found to be involved in gene regulation. It inhibits the activation of some sumoylated transcription factors such as Pax3 and Ets-1 in the nucleus. When recruited to promyelocytic leukemia protein nuclear bodies (PML-NB) through interaction with PML, its transcription repressor activity is alleviated.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

P87A11

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate from NIH3T3 (lane 1, 15 µg), Jurkat (lane 2, 15 µg) and HeLa (lane 3, 15 µg) were resolved by electrophoresis (4-12 % Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-Daxx antibody (clone P87A11). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control.

Conjugate

Unconjugated