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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- R34126 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RACGAP1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids GRQGNFFASPMLK were used as the immunogen for this RACGAP1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the RACGAP1 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A431 nuclear fraction, 2) Jurkat whole cell, 3) Jurkat nuclear fraction and 4) pancreas (negative control) lysate with RACGAP1 antibody at 1ug/ml. Predicted molecular weight: ~71 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF testing of fixed and permeabilized human MCF7 cells with RACGAP1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human testis with RACGAP1 antibody at 4ug/ml. HIER: microwaved with pH9 Tris/EDTA buffer, HRP-staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human MCF7 cells with RACGAP1 antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).
