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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB6112 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6112, RRID:AB_1579502
- Product name
- RACGAP1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of RACGAP1.
- Antigen sequence
C-GRQGNFFASPMLK- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis.
Nishimura Y, Yonemura S
Journal of cell science 2006 Jan 1;119(Pt 1):104-14
Journal of cell science 2006 Jan 1;119(Pt 1):104-14
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RACGAP1 polyclonal antibody (Cat # PAB6112)(1ug/mL) staining of lysate of cell line K-562 (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RACGAP1 polyclonal antibody (Cat # PAB6112) staining (1 ug/mL) of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
