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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00029127-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00029127-M01, RRID:AB_540488
- Product name
- Mouse Monoclonal MgcRacGAP/RACGAP1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RACGAP1 - Rac GTPase activating protein 1
- Reactivity
- Human, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Overexpression of Rac GTPase Activating Protein 1 Contributes to Proliferation of Cancer Cells by Reducing Hippo Signaling to Promote Cytokinesis.
iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
RNA-seq Identification of RACGAP1 as a Metastatic Driver in Uterine Carcinosarcoma.
Rho GTPase Transcriptome Analysis Reveals Oncogenic Roles for Rho GTPase-Activating Proteins in Basal-like Breast Cancers.
Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis.
Upregulation of Rac GTPase-activating protein 1 is significantly associated with the early recurrence of human hepatocellular carcinoma.
Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells.
Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation.
Yang XM, Cao XY, He P, Li J, Feng MX, Zhang YL, Zhang XL, Wang YH, Yang Q, Zhu L, Nie HZ, Jiang SH, Tian GA, Zhang XX, Liu Q, Ji J, Zhu X, Xia Q, Zhang ZG
Gastroenterology 2018 Oct;155(4):1233-1249.e22
Gastroenterology 2018 Oct;155(4):1233-1249.e22
iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
Gao K, Zhang Y, Shi Q, Zhang J, Zhang L, Sun H, Jiao D, Zhao X, Tao H, Wei Y, Wang Y, Saiyin H, Zhao SM, Li Y, Zhang P, Wang C
Cell death & disease 2018 May 1;9(5):528
Cell death & disease 2018 May 1;9(5):528
RNA-seq Identification of RACGAP1 as a Metastatic Driver in Uterine Carcinosarcoma.
Mi S, Lin M, Brouwer-Visser J, Heim J, Smotkin D, Hebert T, Gunter MJ, Goldberg GL, Zheng D, Huang GS
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 Sep 15;22(18):4676-86
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 Sep 15;22(18):4676-86
Rho GTPase Transcriptome Analysis Reveals Oncogenic Roles for Rho GTPase-Activating Proteins in Basal-like Breast Cancers.
Lawson CD, Fan C, Mitin N, Baker NM, George SD, Graham DM, Perou CM, Burridge K, Der CJ, Rossman KL
Cancer research 2016 Jul 1;76(13):3826-37
Cancer research 2016 Jul 1;76(13):3826-37
Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis.
Lekomtsev S, Su KC, Pye VE, Blight K, Sundaramoorthy S, Takaki T, Collinson LM, Cherepanov P, Divecha N, Petronczki M
Nature 2012 Dec 13;492(7428):276-9
Nature 2012 Dec 13;492(7428):276-9
Upregulation of Rac GTPase-activating protein 1 is significantly associated with the early recurrence of human hepatocellular carcinoma.
Wang SM, Ooi LL, Hui KM
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Sep 15;17(18):6040-51
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Sep 15;17(18):6040-51
Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells.
Burkard ME, Maciejowski J, Rodriguez-Bravo V, Repka M, Lowery DM, Clauser KR, Zhang C, Shokat KM, Carr SA, Yaffe MB, Jallepalli PV
PLoS biology 2009 May 5;7(5):e1000111
PLoS biology 2009 May 5;7(5):e1000111
Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation.
Wolfe BA, Takaki T, Petronczki M, Glotzer M
PLoS biology 2009 May 5;7(5):e1000110
PLoS biology 2009 May 5;7(5):e1000110
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Western Blot analysis of RACGAP1 expression in K-562 ( Cat # L009V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in Jurkat.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in 293.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Detection limit for recombinant GST tagged RACGAP1 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Analysis of monoclonal antibody to RACGAP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.
