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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NB100-884 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-884, RRID:AB_2176159
- Product name
- Goat Polyclonal MgcRacGAP/RACGAP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Reported variants represent identical protein: NP_037409.2, NP_001119576.1 and NP_001119575.1.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references CDK11(p58)-cyclin L1β regulates abscission site assembly.
A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation.
A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation.
Renshaw MJ, Panagiotou TC, Lavoie BD, Wilde A
The Journal of biological chemistry 2019 Dec 6;294(49):18639-18649
The Journal of biological chemistry 2019 Dec 6;294(49):18639-18649
A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation.
Reibel L, Dorseuil O, Stancou R, Bertoglio J, Gacon G
Biochemical and biophysical research communications 1991 Mar 15;175(2):451-8
Biochemical and biophysical research communications 1991 Mar 15;175(2):451-8
A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation.
Reibel L, Dorseuil O, Stancou R, Bertoglio J, Gacon G
Biochemical and biophysical research communications 1991 Mar 15;175(2):451-8
Biochemical and biophysical research communications 1991 Mar 15;175(2):451-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MgcRacGAP/RACGAP1 Antibody [NB100-884] - (10ug protein in RIPA buffer) overexpressing Human RACGAP1 with DYKDDDDK tag probed with EB05315 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/500) in lane C. Mock-transfected HEK293 probed with EB05315 (1mg/ml) in Lane B. Prima
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MgcRacGAP/RACGAP1 Antibody [NB100-884] - (1ug/ml) staining of lysate of cell line K562 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MgcRacGAP/RACGAP1 Antibody [NB100-884] - Staining of paraffin embedded Human Testis using this antibody at 1 ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NB100-884] - (4ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MgcRacGAP/RACGAP1 Antibody [NB100-884] - MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
