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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP53556PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53556PU-N, RRID:AB_11145379
- Product name
- anti RACGAP1 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of Human RACGAP1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the RACGAP1 antibody detected the RACGAP1 protein (arrow
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N with MCF-7 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human testis tissue stained with RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of MCF-7 cells using RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N (right histogram) compared to a Negative Control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
