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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA328629 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-Protease-activated Receptor-4 (extracellular)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-Protease-activated Receptor-4 (extracellular)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- F2RL3
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of rat lung (lanes 1 and 3) and testis (lanes 2 and 4) lysates: 1, 2. Anti-Protease-Activated Receptor-4 (extracellular) antibody, (1:200). 3, 4. Anti-Protease-Activated Receptor-4 (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of human prostate carcinoma PC3 (lanes 1 and 4), and LNCaP (lanes 2 and 5), and human T cell leukemia Jurkat (lanes 3 and 6) cell lines: 1-3. Anti-Protease-Activated Receptor-4 (extracellular) antibody, (1:200). 4-6. Anti-Protease-Activated Receptor-4 (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of PAR-4 in a human breast adenocarcinoma cell line. Immunocytochemical staining of a human breast adenocarcinoma cell line. A. Live intact human MCF-7 cells were stained with Anti-Protease-Activated Receptor-4 (extracellular) antibody, (1:50), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of PAR-4 in rat lung. Immunohistochemical staining of rat lung paraffin-embedded sections using Anti-Protease-Activated Receptor-4 (extracellular) antibody , (1:100). Note that staining is present in the respiratory epithelium of the bronchiole (blue arrow) as well as in the smooth muscle cells of the muscularis mucosae (red arrow). Hematoxilin is used as the counterstain.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of PAR-4 in rat epididymis. Immunohistochemical staining of rat epididymis paraffin-embedded sections using Anti-Protease-Activated Receptor-4 (extracellular) antibody, (1:100). Note that strong and specific staining is present in both the pseudostratified epithelium (blue arrow) and the smooth muscle cells of the muscular of blood vessels (red arrow). Hematoxilin is used as the counterstain.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Indirect Flow cytometry analysis of human promyelocytic leukemia HL-60 cells: black line, Unstained cells + FITC-conjugated goat anti-rabbit antibody. green line, Cells + Anti-Protease-Activated Receptor-4 (extracellular) antibody, (1:40) + FITC conjugated goat anti-rabbit antibody.
- Validation comment
- FC
