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Learn15449-1-AP
antibody from Invitrogen Antibodies
Targeting: NSUN5
FLJ10267, NOL1R, NSUN5A, p120(NOL1), WBSCR20, WBSCR20A, Ynl022cL
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- 15449-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSUN5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RNEDLLEVG SRPGPASQLP RFVRVNTLKT CSDDVVDYFK RQGFSYQGRA SSLDDLRALK GKHFLLDPLM PELLVFPAQT DLHEHPLYRA GHLILQDRAS CLPAMLLDPP PGSHVIDACA APGNKTSHLA ALLKNQGKIF AFDLDAKRLA SMATLLARAG VSCCELAEED FLAVSPSDPR YHEVHYILLD PSCSGSGMPS RQLEEPGAGT PSPVRLHALA GFQQRALCHA LTFPSLQRLV YSTCSLCQEE NEDVVRDALQ QNPGAFRLAP ALPAWPHRGL STFPGAEHCL RASPETTLSS GFFVAVIERV EVPSSASQAK ASAPERTPSP APKRKKRQQR AAAGACTPPC T (117-466 aa encoded by BC008084)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human kidney tissue were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using NSUN5 antibody 15449-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer using 15449-1-AP (NSUN5 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer using 15449-1-AP (NSUN5 antibody) at dilution of 1:50 (under 40x lens).
