H00005463-M01

antibody from Abnova Corporation

Targeting: POU6F1 BRN5, MPOU, TCFB1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005463-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005463-M01, RRID:AB_606812

Product name

POU6F1 monoclonal antibody (M01), clone 6H1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant POU6F1.

Antigen sequence

ITPKSAQKLKPVLEKWLNEAELRNQEGQQNLMEFV
GGEPSKKRKRRTSFTPQAIEALNAYFEKNPLPTGQ
EITEIAKELNYDREVVRVWFCNRRQTLKNTSKLNV
FQIP


Isotype

IgG

Antibody clone number

6H1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

POU6F1 monoclonal antibody (M01), clone 6H1 Western Blot analysis of POU6F1 expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of POU6F1 expression in transfected 293T cell line by POU6F1 monoclonal antibody (M01), clone 6H1.Lane 1: POU6F1 transfected lysate(32.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged POU6F1 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol