Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB7314 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7314, RRID:AB_1574587
- Product name
- EGR2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of EGR2.
- Antigen sequence
HGTAGPDRKPFPC
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Neuropathy-associated Egr2 mutants disrupt cooperative activation of myelin protein zero by Egr2 and Sox10.
LeBlanc SE, Ward RM, Svaren J
Molecular and cellular biology 2007 May;27(9):3521-9
Molecular and cellular biology 2007 May;27(9):3521-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EGR2 polyclonal antibody (Cat # PAB7314) (0.03 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EGR2 polyclonal antibody (Cat # PAB7314, 3.8 ug/mL) staining of paraffin embedded human colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)