PA5-96137

antibody from Invitrogen Antibodies

Targeting: SIAH1 hSIAH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96137

Provider

Invitrogen Antibodies

Product name

SIAH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SLDAVMPHLM HQHKSITTLQ GEDIVFLATD INLPGAVDWV MMQSCFGFHF MLVLEKQEKY DGHQQFFAIV QLIGTRKQAE NFAYRLELNG HRRRLTWEAT PRSIHEGIAT AIMNSDCLVF DTSIAQLFAE NGNLGINVTI SMC

Concentration

1.03 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SIAH1 in extracts of mouse kidney using SIAH1 Polyclonal Antibody (Product # PA5-96137) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SIAH1 was performed in U2OS cells using SIAH1 Polyclonal Antibody (Product # PA5-96137).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SIAH1 in paraffin-embedded rat brain using SIAH1 Polyclonal Antibody (Product # PA5-96137) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SIAH1 in paraffin-embedded human colon carcinoma using SIAH1 Polyclonal Antibody (Product # PA5-96137) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SIAH1 in paraffin-embedded mouse spleen using SIAH1 Polyclonal Antibody (Product # PA5-96137) at a dilution of 1:200.