14662-1-AP

antibody from Invitrogen Antibodies

Targeting: AFF4 AF5Q31, MCEF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14662-1-AP

Provider

Invitrogen Antibodies

Product name

AFF4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MNREDRNVL RMKERERRNQ EIQQGEDAFP PSSPLFAEPY KVTSKEDKLS SRIQSMLGNY DEMKDFIGDR SIPKLVAIPK PTVPPSADEK SNPNFFEQRH GGSHQSSKWT PVGPAPSTSQ SQKRSSGLQS GHSSQRTSAG SSSGTNSSGQ RHDRESYNNS GSSSRKKGQH GSEHSKSRSS SPGKPQAVSS LNSSHSRSHG NDHHSKEHQR SKSPRDPDAN WDSPSRVPFS SGQHSTQSFP PSLMSKSNSM LQKPTAYVRP MDGQESMEPK LSSEHYSSQS HGNSMTELKP SSKAHLTKLK IPSQPLDASA SGDVSCVDEI LKEMTHSWPP PLTAIHTPC (1-338 aa encoded by BC063007 )

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.15 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14662-1-AP (AFF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14662-1-AP (AFF4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 14662-1-AP (AFF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed L02 cells using 14662-1-AP (AFF4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-AFF4 (IP:14662-1-AP, 4ug; Detection:14662-1-AP 1:300) with HepG2 cells lysate 3200ug.