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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- F50035 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PKC nu Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 352-384 from the human protein was used as the immunogen for this PKC nu antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the PKC nu antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of PKC nu antibody in lysate of HL-60 cells stimulated with PMA.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- A. Western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: PKD-transfected cells; lanes 3 and 7: PKD2-transfected cells; lanes 4 and 8: PKC nu transfected cells. B. Western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKC nu antibodies. C. Indirect immunofluorescence analysis of PKC nu fusion protein expression in Panc-1 cells. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human breast carcinoma tissue stained with the PKC nu antibody
