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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB4544 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4544, RRID:AB_1579161
- Product name
- PRKD3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRKD3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Protein kinase D3 (PKD3) contributes to prostate cancer cell growth and survival through a PKCepsilon/PKD3 pathway downstream of Akt and ERK 1/2.
Chen J, Deng F, Singh SV, Wang QJ
Cancer research 2008 May 15;68(10):3844-53
Cancer research 2008 May 15;68(10):3844-53
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an PRKD3 polyclonal antibody (Cat # PAB4544) .Lanes 1 and 5 : non-transfected cells;lanes 2 and 6 : GFP-PRKD-transfected cells;lanes 3 and 7 : GFP-PRKD2-transfected cells;lanes 4 and 8 : GFP-PRKD3 transfected cells .Lower panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PRKD3 polyclonal antibody (Cat # PAB4544) and PRKD3 polyclonal antibody (Cat # PAB4545) .
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRKD3 polyclonal antibody (Cat # PAB4544) in lysate of HL-60 cells stimulated with PMA (Lane A) and mouse brain tissue lysate (Lane B). PRKD3 (arrow) was detected using purified PRKD3 polyclonal antibody (Cat # PAB4544). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Indirect immunofluorescence analysis of GFP-PRKD3 fusion protein expression in Panc-1 cells by using PRKD3 polyclonal antibody (Cat # PAB4544) and PRKD3 polyclonal antibody (Cat # PAB4545) . Data courtesy of Dr . Osvaldo Rey, University of California Los Angeles .
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PRKD3 polyclonal antibody (Cat # PAB4544) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
