Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB4545 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4545, RRID:AB_1579160
- Product name
- PRKD3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRKD3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network.
Regulation of protein kinase Cnu by the B-cell antigen receptor.
Protein kinase C nu/protein kinase D3 nuclear localization, catalytic activation, and intracellular redistribution in response to G protein-coupled receptor agonists.
Yeaman C, Ayala MI, Wright JR, Bard F, Bossard C, Ang A, Maeda Y, Seufferlein T, Mellman I, Nelson WJ, Malhotra V
Nature cell biology 2004 Feb;6(2):106-12
Nature cell biology 2004 Feb;6(2):106-12
Regulation of protein kinase Cnu by the B-cell antigen receptor.
Matthews SA, Dayalu R, Thompson LJ, Scharenberg AM
The Journal of biological chemistry 2003 Mar 14;278(11):9086-91
The Journal of biological chemistry 2003 Mar 14;278(11):9086-91
Protein kinase C nu/protein kinase D3 nuclear localization, catalytic activation, and intracellular redistribution in response to G protein-coupled receptor agonists.
Rey O, Yuan J, Young SH, Rozengurt E
The Journal of biological chemistry 2003 Jun 27;278(26):23773-85
The Journal of biological chemistry 2003 Jun 27;278(26):23773-85
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an PRKD3 polyclonal antibody (Cat # PAB4545) . Lanes 1 and 5 : non-transfected cells;lanes 2 and 6 : GFP-PRKD-transfected cells;lanes 3 and 7 : GFP-PRKD2-transfected cells;lanes 4 and 8 : GFP-PRKD3 transfected cells . Lower panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PRKD3 polyclonal antibody (Cat # PAB4544) and PRKD3 polyclonal antibody (Cat # PAB4545) .
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRKD3 polyclonal antibody (Cat # PAB4545) in NCI-H460 cell lysate . PRKD3 (arrow) was detected using purified PRKD3 polyclonal antibody (Cat # PAB4545) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Indirect immunofluorescence analysis of GFP-PRKD3 fusion protein expression in Panc-1 cells by using PRKD3 polyclonal antibody (Cat # PAB4544) and PRKD3 polyclonal antibody (Cat # PAB4545) . Data courtesy of Dr . Osvaldo Rey, University of California Los Angeles .
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the PRKD3 polyclonal antibody (Cat # PAB4545) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
