GTX25286

antibody from GeneTex

Targeting: PRKD3 EPK2, PRKCN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX25286

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25286, RRID:AB_380496

Product name

PKC nu antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of anti-PKCnu C-term Pab (GTX25286) in NCI-H460 cell lysate. PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

"Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lane 5: non-transfected cells Lane 6: GFP-PKD-transfected cells Lane 7: GFP-PKD2-transfected cells Lane 8: GFP-PKD3 transfected cells Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 C-term (GTX25286) antibody. "

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using PKD3 C-term (GTX25286) antibody.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary PKD3 C-term (GTX25286) antibody, which was peroxidase-conjμgated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry.HC = heptaocarcinoma