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LearnNB400-137
antibody from Novus Biologicals
Targeting: MAFA
hMafA, RIPE3b1
Western blot
Immunocytochemistry Immunohistochemistry Gel shift Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [42]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- NB400-137 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB400-137, RRID:AB_10002142
- Product name
- Rabbit Polyclonal MafA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Vitamin D Switches BAF Complexes to Protect β Cells.
Origin and Segmental Diversity of Spinal Inhibitory Interneurons.
Constitutive role of GADD34 and CReP in cancellation of phospho-eIF2α-dependent translational attenuation and insulin biosynthesis in pancreatic β cells.
Feedback inhibition of CREB signaling promotes beta cell dysfunction in insulin resistance.
Maternal diet-induced microRNAs and mTOR underlie β cell dysfunction in offspring.
Identification of multiple subsets of ventral interneurons and differential distribution along the rostrocaudal axis of the developing spinal cord.
Staining protocols for human pancreatic islets.
c-Maf is required for the development of dorsal horn laminae III/IV neurons and mechanoreceptive DRG axon projections.
MafA and MafB regulate genes critical to beta-cells in a unique temporal manner.
Vagal control of pancreatic ß-cell proliferation.
The Krüppel-like zinc finger protein Glis3 directly and indirectly activates insulin gene transcription.
The stability and transactivation potential of the mammalian MafA transcription factor are regulated by serine 65 phosphorylation.
Transcription factor Glis3, a novel critical player in the regulation of pancreatic beta-cell development and insulin gene expression.
Neither MafA/L-Maf nor MafB is essential for lens development in mice.
In vivo reprogramming of adult pancreatic exocrine cells to beta-cells.
Elevated hepatic fatty acid elongase-5 activity affects multiple pathways controlling hepatic lipid and carbohydrate composition.
Role of the transcription factor sox4 in insulin secretion and impaired glucose tolerance.
PANIC-ATTAC: a mouse model for inducible and reversible beta-cell ablation.
MafA is a dedicated activator of the insulin gene in vivo.
Endoplasmic reticulum stress-induced activation of activating transcription factor 6 decreases insulin gene expression via up-regulation of orphan nuclear receptor small heterodimer partner.
MafA and MafB regulate Pdx1 transcription through the Area II control region in pancreatic beta cells.
MafA regulates expression of genes important to islet beta-cell function.
MafB is required for islet beta cell maturation.
Conserved markers of fetal pancreatic epithelium permit prospective isolation of islet progenitor cells by FACS.
Glucotoxicity in the INS-1 rat insulinoma cell line is mediated by the orphan nuclear receptor small heterodimer partner.
Nkx2.2-repressor activity is sufficient to specify alpha-cells and a small number of beta-cells in the pancreatic islet.
Nkx2.2 regulates beta-cell function in the mature islet.
Marked increase of insulin gene transcription by suppression of the Rho/Rho-kinase pathway.
Total insulin and IGF-I resistance in pancreatic beta cells causes overt diabetes.
Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies.
MafA expression and insulin promoter activity are induced by nicotinamide and related compounds in INS-1 pancreatic beta-cells.
MafA expression and insulin promoter activity are induced by nicotinamide and related compounds in INS-1 pancreatic beta-cells.
Conditional expression of Smad7 in pancreatic beta cells disrupts TGF-beta signaling and induces reversible diabetes mellitus.
Maintenance of hepatic nuclear factor 6 in postnatal islets impairs terminal differentiation and function of beta-cells.
MafK overexpression in pancreatic beta-cells caused impairment of glucose-stimulated insulin secretion.
Palmitate inhibits insulin gene expression by altering PDX-1 nuclear localization and reducing MafA expression in isolated rat islets of Langerhans.
Elevated glucose attenuates human insulin gene promoter activity in INS-1 pancreatic beta-cells via reduced nuclear factor binding to the A5/core and Z element.
The islet beta cell-enriched MafA activator is a key regulator of insulin gene transcription.
Oxidative stress-mediated, post-translational loss of MafA protein as a contributing mechanism to loss of insulin gene expression in glucotoxic beta cells.
The MafA transcription factor appears to be responsible for tissue-specific expression of insulin.
Members of the large Maf transcription family regulate insulin gene transcription in islet beta cells.
Members of the large Maf transcription family regulate insulin gene transcription in islet beta cells.
Wei Z, Yoshihara E, He N, Hah N, Fan W, Pinto AFM, Huddy T, Wang Y, Ross B, Estepa G, Dai Y, Ding N, Sherman MH, Fang S, Zhao X, Liddle C, Atkins AR, Yu RT, Downes M, Evans RM
Cell 2018 May 17;173(5):1135-1149.e15
Cell 2018 May 17;173(5):1135-1149.e15
Origin and Segmental Diversity of Spinal Inhibitory Interneurons.
Sweeney LB, Bikoff JB, Gabitto MI, Brenner-Morton S, Baek M, Yang JH, Tabak EG, Dasen JS, Kintner CR, Jessell TM
Neuron 2018 Jan 17;97(2):341-355.e3
Neuron 2018 Jan 17;97(2):341-355.e3
Constitutive role of GADD34 and CReP in cancellation of phospho-eIF2α-dependent translational attenuation and insulin biosynthesis in pancreatic β cells.
Akai R, Hosoda A, Yoshino M, Iwawaki T
Genes to cells : devoted to molecular & cellular mechanisms 2015 Nov;20(11):871-86
Genes to cells : devoted to molecular & cellular mechanisms 2015 Nov;20(11):871-86
Feedback inhibition of CREB signaling promotes beta cell dysfunction in insulin resistance.
Blanchet E, Van de Velde S, Matsumura S, Hao E, LeLay J, Kaestner K, Montminy M
Cell reports 2015 Feb 24;10(7):1149-57
Cell reports 2015 Feb 24;10(7):1149-57
Maternal diet-induced microRNAs and mTOR underlie β cell dysfunction in offspring.
Alejandro EU, Gregg B, Wallen T, Kumusoglu D, Meister D, Chen A, Merrins MJ, Satin LS, Liu M, Arvan P, Bernal-Mizrachi E
The Journal of clinical investigation 2014 Oct;124(10):4395-410
The Journal of clinical investigation 2014 Oct;124(10):4395-410
Identification of multiple subsets of ventral interneurons and differential distribution along the rostrocaudal axis of the developing spinal cord.
Francius C, Harris A, Rucchin V, Hendricks TJ, Stam FJ, Barber M, Kurek D, Grosveld FG, Pierani A, Goulding M, Clotman F
PloS one 2013;8(8):e70325
PloS one 2013;8(8):e70325
Staining protocols for human pancreatic islets.
Campbell-Thompson ML, Heiple T, Montgomery E, Zhang L, Schneider L
Journal of visualized experiments : JoVE 2012 May 23;(63):e4068
Journal of visualized experiments : JoVE 2012 May 23;(63):e4068
c-Maf is required for the development of dorsal horn laminae III/IV neurons and mechanoreceptive DRG axon projections.
Hu J, Huang T, Li T, Guo Z, Cheng L
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Apr 18;32(16):5362-73
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Apr 18;32(16):5362-73
MafA and MafB regulate genes critical to beta-cells in a unique temporal manner.
Artner I, Hang Y, Mazur M, Yamamoto T, Guo M, Lindner J, Magnuson MA, Stein R
Diabetes 2010 Oct;59(10):2530-9
Diabetes 2010 Oct;59(10):2530-9
Vagal control of pancreatic ß-cell proliferation.
Lausier J, Diaz WC, Roskens V, LaRock K, Herzer K, Fong CG, Latour MG, Peshavaria M, Jetton TL
American journal of physiology. Endocrinology and metabolism 2010 Nov;299(5):E786-93
American journal of physiology. Endocrinology and metabolism 2010 Nov;299(5):E786-93
The Krüppel-like zinc finger protein Glis3 directly and indirectly activates insulin gene transcription.
Yang Y, Chang BH, Samson SL, Li MV, Chan L
Nucleic acids research 2009 May;37(8):2529-38
Nucleic acids research 2009 May;37(8):2529-38
The stability and transactivation potential of the mammalian MafA transcription factor are regulated by serine 65 phosphorylation.
Guo S, Burnette R, Zhao L, Vanderford NL, Poitout V, Hagman DK, Henderson E, Ozcan S, Wadzinski BE, Stein R
The Journal of biological chemistry 2009 Jan 9;284(2):759-65
The Journal of biological chemistry 2009 Jan 9;284(2):759-65
Transcription factor Glis3, a novel critical player in the regulation of pancreatic beta-cell development and insulin gene expression.
Kang HS, Kim YS, ZeRuth G, Beak JY, Gerrish K, Kilic G, Sosa-Pineda B, Jensen J, Pierreux CE, Lemaigre FP, Foley J, Jetten AM
Molecular and cellular biology 2009 Dec;29(24):6366-79
Molecular and cellular biology 2009 Dec;29(24):6366-79
Neither MafA/L-Maf nor MafB is essential for lens development in mice.
Takeuchi T, Kudo T, Ogata K, Hamada M, Nakamura M, Kito K, Abe Y, Ueda N, Yamamoto M, Engel JD, Takahashi S
Genes to cells : devoted to molecular & cellular mechanisms 2009 Aug;14(8):941-7
Genes to cells : devoted to molecular & cellular mechanisms 2009 Aug;14(8):941-7
In vivo reprogramming of adult pancreatic exocrine cells to beta-cells.
Zhou Q, Brown J, Kanarek A, Rajagopal J, Melton DA
Nature 2008 Oct 2;455(7213):627-32
Nature 2008 Oct 2;455(7213):627-32
Elevated hepatic fatty acid elongase-5 activity affects multiple pathways controlling hepatic lipid and carbohydrate composition.
Wang Y, Torres-Gonzalez M, Tripathy S, Botolin D, Christian B, Jump DB
Journal of lipid research 2008 Jul;49(7):1538-52
Journal of lipid research 2008 Jul;49(7):1538-52
Role of the transcription factor sox4 in insulin secretion and impaired glucose tolerance.
Goldsworthy M, Hugill A, Freeman H, Horner E, Shimomura K, Bogani D, Pieles G, Mijat V, Arkell R, Bhattacharya S, Ashcroft FM, Cox RD
Diabetes 2008 Aug;57(8):2234-44
Diabetes 2008 Aug;57(8):2234-44
PANIC-ATTAC: a mouse model for inducible and reversible beta-cell ablation.
Wang ZV, Mu J, Schraw TD, Gautron L, Elmquist JK, Zhang BB, Brownlee M, Scherer PE
Diabetes 2008 Aug;57(8):2137-48
Diabetes 2008 Aug;57(8):2137-48
MafA is a dedicated activator of the insulin gene in vivo.
Artner I, Hang Y, Guo M, Gu G, Stein R
The Journal of endocrinology 2008 Aug;198(2):271-9
The Journal of endocrinology 2008 Aug;198(2):271-9
Endoplasmic reticulum stress-induced activation of activating transcription factor 6 decreases insulin gene expression via up-regulation of orphan nuclear receptor small heterodimer partner.
Seo HY, Kim YD, Lee KM, Min AK, Kim MK, Kim HS, Won KC, Park JY, Lee KU, Choi HS, Park KG, Lee IK
Endocrinology 2008 Aug;149(8):3832-41
Endocrinology 2008 Aug;149(8):3832-41
MafA and MafB regulate Pdx1 transcription through the Area II control region in pancreatic beta cells.
Vanhoose AM, Samaras S, Artner I, Henderson E, Hang Y, Stein R
The Journal of biological chemistry 2008 Aug 15;283(33):22612-9
The Journal of biological chemistry 2008 Aug 15;283(33):22612-9
MafA regulates expression of genes important to islet beta-cell function.
Matsuoka TA, Kaneto H, Stein R, Miyatsuka T, Kawamori D, Henderson E, Kojima I, Matsuhisa M, Hori M, Yamasaki Y
Molecular endocrinology (Baltimore, Md.) 2007 Nov;21(11):2764-74
Molecular endocrinology (Baltimore, Md.) 2007 Nov;21(11):2764-74
MafB is required for islet beta cell maturation.
Artner I, Blanchi B, Raum JC, Guo M, Kaneko T, Cordes S, Sieweke M, Stein R
Proceedings of the National Academy of Sciences of the United States of America 2007 Mar 6;104(10):3853-8
Proceedings of the National Academy of Sciences of the United States of America 2007 Mar 6;104(10):3853-8
Conserved markers of fetal pancreatic epithelium permit prospective isolation of islet progenitor cells by FACS.
Sugiyama T, Rodriguez RT, McLean GW, Kim SK
Proceedings of the National Academy of Sciences of the United States of America 2007 Jan 2;104(1):175-80
Proceedings of the National Academy of Sciences of the United States of America 2007 Jan 2;104(1):175-80
Glucotoxicity in the INS-1 rat insulinoma cell line is mediated by the orphan nuclear receptor small heterodimer partner.
Park KG, Lee KM, Seo HY, Suh JH, Kim HS, Wang L, Won KC, Lee HW, Park JY, Lee KU, Kim JG, Kim BW, Choi HS, Lee IK
Diabetes 2007 Feb;56(2):431-7
Diabetes 2007 Feb;56(2):431-7
Nkx2.2-repressor activity is sufficient to specify alpha-cells and a small number of beta-cells in the pancreatic islet.
Doyle MJ, Loomis ZL, Sussel L
Development (Cambridge, England) 2007 Feb;134(3):515-23
Development (Cambridge, England) 2007 Feb;134(3):515-23
Nkx2.2 regulates beta-cell function in the mature islet.
Doyle MJ, Sussel L
Diabetes 2007 Aug;56(8):1999-2007
Diabetes 2007 Aug;56(8):1999-2007
Marked increase of insulin gene transcription by suppression of the Rho/Rho-kinase pathway.
Nakamura Y, Kaneto H, Miyatsuka T, Matsuoka TA, Matsuhisa M, Node K, Hori M, Yamasaki Y
Biochemical and biophysical research communications 2006 Nov 10;350(1):68-73
Biochemical and biophysical research communications 2006 Nov 10;350(1):68-73
Total insulin and IGF-I resistance in pancreatic beta cells causes overt diabetes.
Ueki K, Okada T, Hu J, Liew CW, Assmann A, Dahlgren GM, Peters JL, Shackman JG, Zhang M, Artner I, Satin LS, Stein R, Holzenberger M, Kennedy RT, Kahn CR, Kulkarni RN
Nature genetics 2006 May;38(5):583-8
Nature genetics 2006 May;38(5):583-8
Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies.
Wichroski MJ, Robb GB, Rana TM
PLoS pathogens 2006 May;2(5):e41
PLoS pathogens 2006 May;2(5):e41
MafA expression and insulin promoter activity are induced by nicotinamide and related compounds in INS-1 pancreatic beta-cells.
Ye DZ, Tai MH, Linning KD, Szabo C, Olson LK
Diabetes 2006 Mar;55(3):742-50
Diabetes 2006 Mar;55(3):742-50
MafA expression and insulin promoter activity are induced by nicotinamide and related compounds in INS-1 pancreatic beta-cells.
Ye DZ, Tai MH, Linning KD, Szabo C, Olson LK
Diabetes 2006 Mar;55(3):742-50
Diabetes 2006 Mar;55(3):742-50
Conditional expression of Smad7 in pancreatic beta cells disrupts TGF-beta signaling and induces reversible diabetes mellitus.
Smart NG, Apelqvist AA, Gu X, Harmon EB, Topper JN, MacDonald RJ, Kim SK
PLoS biology 2006 Feb;4(2):e39
PLoS biology 2006 Feb;4(2):e39
Maintenance of hepatic nuclear factor 6 in postnatal islets impairs terminal differentiation and function of beta-cells.
Tweedie E, Artner I, Crawford L, Poffenberger G, Thorens B, Stein R, Powers AC, Gannon M
Diabetes 2006 Dec;55(12):3264-70
Diabetes 2006 Dec;55(12):3264-70
MafK overexpression in pancreatic beta-cells caused impairment of glucose-stimulated insulin secretion.
Shimohata H, Yoh K, Morito N, Shimano H, Kudo T, Takahashi S
Biochemical and biophysical research communications 2006 Aug 4;346(3):671-80
Biochemical and biophysical research communications 2006 Aug 4;346(3):671-80
Palmitate inhibits insulin gene expression by altering PDX-1 nuclear localization and reducing MafA expression in isolated rat islets of Langerhans.
Hagman DK, Hays LB, Parazzoli SD, Poitout V
The Journal of biological chemistry 2005 Sep 16;280(37):32413-8
The Journal of biological chemistry 2005 Sep 16;280(37):32413-8
Elevated glucose attenuates human insulin gene promoter activity in INS-1 pancreatic beta-cells via reduced nuclear factor binding to the A5/core and Z element.
Pino MF, Ye DZ, Linning KD, Green CD, Wicksteed B, Poitout V, Olson LK
Molecular endocrinology (Baltimore, Md.) 2005 May;19(5):1343-60
Molecular endocrinology (Baltimore, Md.) 2005 May;19(5):1343-60
The islet beta cell-enriched MafA activator is a key regulator of insulin gene transcription.
Zhao L, Guo M, Matsuoka TA, Hagman DK, Parazzoli SD, Poitout V, Stein R
The Journal of biological chemistry 2005 Mar 25;280(12):11887-94
The Journal of biological chemistry 2005 Mar 25;280(12):11887-94
Oxidative stress-mediated, post-translational loss of MafA protein as a contributing mechanism to loss of insulin gene expression in glucotoxic beta cells.
Harmon JS, Stein R, Robertson RP
The Journal of biological chemistry 2005 Mar 25;280(12):11107-13
The Journal of biological chemistry 2005 Mar 25;280(12):11107-13
The MafA transcription factor appears to be responsible for tissue-specific expression of insulin.
Matsuoka TA, Artner I, Henderson E, Means A, Sander M, Stein R
Proceedings of the National Academy of Sciences of the United States of America 2004 Mar 2;101(9):2930-3
Proceedings of the National Academy of Sciences of the United States of America 2004 Mar 2;101(9):2930-3
Members of the large Maf transcription family regulate insulin gene transcription in islet beta cells.
Matsuoka TA, Zhao L, Artner I, Jarrett HW, Friedman D, Means A, Stein R
Molecular and cellular biology 2003 Sep;23(17):6049-62
Molecular and cellular biology 2003 Sep;23(17):6049-62
Members of the large Maf transcription family regulate insulin gene transcription in islet beta cells.
Matsuoka TA, Zhao L, Artner I, Jarrett HW, Friedman D, Means A, Stein R
Molecular and cellular biology 2003 Sep;23(17):6049-62
Molecular and cellular biology 2003 Sep;23(17):6049-62
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MafA Antibody [NB400-137] - Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody (NB 400-137).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MafA Antibody [NB400-137] - Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Affinity purified anti-MafA antibody (NB400-137), anti-MafB antibody (NB600-266) or anti-c-Maf antibody (NB600-267). Each antibody was used at 0.5 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MafA Antibody [NB400-137] - Immunohistochemical staining of MafA (blue) and insulin (green) in mouse pancreas. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Gel Super Shift Assays: MafA Antibody [NB400-137] - Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody NB400-137, anti-MafB antibody NB600-266 or anti-c-Maf antibody NB600-267.
