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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB28732 - Provider product page
- Provider
- Abnova Corporation
- Product name
- BEGAIN polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant BEGAIN.
- Antigen sequence
PYPAETFRFPASPGPQQALMPPNLWSLRAKPGTAR
LPGEDMRGQWRPLSVEDIGAYSYPVSAAGRASPCS
FSERYYGGAGGSPGKKADGRASPLYASYKADSFSE
GDDLSQGHLAEPCFLRAGGDLSLSPGRSADPLPGY
APSE- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412260) with BEGAIN polyclonal antibody (Cat # PAB28732) at 1:100-1:250 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line A-431 with BEGAIN polyclonal antibody (Cat # PAB28732) at 1-4 ug/mL shows positivity in nucleus but not nucleoli and golgi apparatus.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human stomach with BEGAIN polyclonal antibody (Cat # PAB28732) shows strong cytoplasmic positivity in granular pattern in glandular cells at 1:200-1:500 dilution.
- Validation comment
- Immunohistochemistry
