Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- sc-15334 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-15334, RRID:AB_2146667
- Product name
- Anti-MUC2
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Sox9 expression marks a subset of CD24-expressing small intestine epithelial stem cells that form organoids in vitro.
Gene expression profiling of metaplastic lineages identifies CDH17 as a prognostic marker in early stage gastric cancer.
Sulfation of colonic mucins by N-acetylglucosamine 6-O-sulfotransferase-2 and its protective function in experimental colitis in mice.
The protein disulfide isomerase AGR2 is essential for production of intestinal mucus.
Multilayered epithelium in a rat model and human Barrett's esophagus: similar expression patterns of transcription factors and differentiation markers.
Combined defects in epithelial and immunoregulatory factors exacerbate the pathogenesis of inflammation: mucin 2-interleukin 10-deficient mice
Gracz AD, Ramalingam S, Magness ST
American journal of physiology. Gastrointestinal and liver physiology 2010 May;298(5):G590-600
American journal of physiology. Gastrointestinal and liver physiology 2010 May;298(5):G590-600
Gene expression profiling of metaplastic lineages identifies CDH17 as a prognostic marker in early stage gastric cancer.
Lee HJ, Nam KT, Park HS, Kim MA, Lafleur BJ, Aburatani H, Yang HK, Kim WH, Goldenring JR
Gastroenterology 2010 Jul;139(1):213-25.e3
Gastroenterology 2010 Jul;139(1):213-25.e3
Sulfation of colonic mucins by N-acetylglucosamine 6-O-sulfotransferase-2 and its protective function in experimental colitis in mice.
Tobisawa Y, Imai Y, Fukuda M, Kawashima H
The Journal of biological chemistry 2010 Feb 26;285(9):6750-60
The Journal of biological chemistry 2010 Feb 26;285(9):6750-60
The protein disulfide isomerase AGR2 is essential for production of intestinal mucus.
Park SW, Zhen G, Verhaeghe C, Nakagami Y, Nguyenvu LT, Barczak AJ, Killeen N, Erle DJ
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 28;106(17):6950-5
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 28;106(17):6950-5
Multilayered epithelium in a rat model and human Barrett's esophagus: similar expression patterns of transcription factors and differentiation markers.
Chen X, Qin R, Liu B, Ma Y, Su Y, Yang CS, Glickman JN, Odze RD, Shaheen NJ
BMC gastroenterology 2008 Jan 11;8:1
BMC gastroenterology 2008 Jan 11;8:1
Combined defects in epithelial and immunoregulatory factors exacerbate the pathogenesis of inflammation: mucin 2-interleukin 10-deficient mice
Maria van der Sluis, Janneke Bouma, Audrey Vincent, Anna Velcich, Kermit L Carraway, Hans A Büller, Alexandra W C Einerhand, Johannes B van Goudoever, Isabelle Van Seuningen, Ingrid B Renes
Laboratory Investigation 2008 Apr;88(6):634-642
Laboratory Investigation 2008 Apr;88(6):634-642
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 28
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
