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LearnH00009569-M08
antibody from Abnova Corporation
Targeting: GTF2IRD1
BEN, Cream1, GTF3, MusTRD1, RBAP2, WBSCR11, WBSCR12
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00009569-M08 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009569-M08, RRID:AB_1674844
- Product name
- GTF2IRD1 monoclonal antibody (M08), clone 2C7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant GTF2IRD1.
- Antigen sequence
MALLGKRCDVPTNGCGPDRWNSAFTRKDEIITSLV
SALDSMCSALSKLNAEVACVAVHDESAFVVSTEKG
RMFLNARKELQSDFLRFCRGPPWKDPEAEHPKKVQ
RGEGGGRSLPRSSLEHGSDVYLLRKMVEEVFDVLY
SEALGRASVVPLPYERLLREPGLLAVQGLPEGLAF
RRPAEYDPKALMAILEHSHRIRFKLKRPLEDGGRD
SKALVELNGVSLIPKGSRDCGLHGQAPKVPPQDLP
PTATSSSMASFLYSTALPNHAIRELKQEAPSCPLA
PSDLGLSRPMPEPKATGAQDFSDCCGQKPTGPGGP
LIQNVHASKRILFSIVHDKSEKWDAFIKETEDINT
LRECVQILFNSRYAEALGLDHMVPVPYRKIACDPE
AVEIVGIPDKIPFKRPCTYGVPKLKRILEERHSIH
FIIKRMFDERIFTGNKFTKDTTKLEPASPPEDTSA
EVSRATVLDLAGNARSDKGSMSEDCGPGTSGELGG
LRPIKIEPEDLDIIQVTVPDPSPTSEEMTDSMPGH
LPSEDSGYGMEMLTDKGLSEDARPEERPVEDSHGD
VIRPLRKQVELLFNTRYAKAIGISEPVKVPYSKFL
MHPEELFVVGLPEGISLRRPNCFGIAKLRKILEAS
NSIQFVIKRPELLTEGVKEPIVDSQGTASSLGFSP
PALPPERDSGDPLVDESLKRQGFQENYDARLSRID
IANTLREQVQDLFNKKYGEALGIKYPVQVPYKRIK
SNPGSVIIEGLPPGIPFRKPCTFGSQNLERILAVA
DKIKFTVTRPFQGLIPKPDEDDANRLGEKVILREQ
VKELFNEKYGEALGLNRPVLVPYKLIRDSPDAVEV
TGLPDDIPFRNPNTYDIHRLEKILKAREHVRMVII
NQLQPFAEICNDAKVPAKDSSIPKRKRKRVSEGNS
VSSSSSSSSSSSSNPDSVASANQISFVQWPMYMVD
YAGLNVQLPGPLNY- Isotype
- IgG
- Antibody clone number
- 2C7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of GTF2IRD1 expression in transfected 293T cell line by GTF2IRD1 monoclonal antibody (M08), clone 2C7.Lane 1: GTF2IRD1 transfected lysate (Predicted MW: 106 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged GTF2IRD1 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to GTF2IRD1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
