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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB18281 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB18281, RRID:AB_10717146
- Product name
- PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues surrounding T497 of human PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
Wissing J, Jänsch L, Nimtz M, Dieterich G, Hornberger R, Kéri G, Wehland J, Daub H
Molecular & cellular proteomics : MCP 2007 Mar;6(3):537-47
Molecular & cellular proteomics : MCP 2007 Mar;6(3):537-47
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J
Nature biotechnology 2003 May;21(5):566-9
Nature biotechnology 2003 May;21(5):566-9
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250 ng/mL, 15 mins), using PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ polyclonal antibody (Cat # PAB18281).Peptide "+" means "peptide blocking".
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HUVEC cells, using PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ polyclonal antibody (Cat # PAB18281).Peptide "+" means "peptide blocking".
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ polyclonal antibody (Cat # PAB18281).Peptide "+" means "peptide blocking".
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
