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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM05346PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM05346PU-N, RRID:AB_1609575
- Product name
- anti Alpha-Synuclein / SNCA
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human Alpha-Synuclein.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- Syn 211
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Extracts of SHSY-5Y human neuroblastoma cells were resolved by SDS PAGE and transferred to a PVDF membrane. Membranes were incubated with 1 μg/mL of the anti-α synuclein antibody (Syn 211). After washing, membranes were incubated with goat F(abâ)2 anti Mouse IgG alkaline phosphatase diluted 1:5000 and the membrane was incubated with CDP-Substrate using the WesternStarTM method (Tropix). The membrane was then exposed to Kodak BioMax film.
