Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB13381 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat alpha-Synuclein Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human alpha-Synuclein in direct ELISAs and human, mouse and rat alpha-Synuclein in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P37840- Isotype
- IgG
- Antibody clone number
- 913611
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat alpha-Synuclein by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, and rat brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat alpha-Synuclein Monoclonal Antibody (Catalog # MAB13381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- alpha-Synuclein in Human Brain. alpha-Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human/Mouse/Rat alpha-Synuclein Monoclonal Antibody (Catalog # MAB13381) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic varicosities. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
