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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP22453PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP22453PU-N, RRID:AB_10758209
- Product name
- anti SS-A / Ro52 / TRIM21 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide (CPLNIGSQGSTDY) from C-terminus of Human TRIM21
- Reactivity
- Human
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with AP22453PU-N TRIM21 antibody (1 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in Lane C. Mock-transfected HEK293 probed with AP22453PU-N (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP22453PU-N TRIM21 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Colon at 5 µg/ml using Biotin conjugated anti-Goat IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
