Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF6219 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TRIM21 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TRIM21 in Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P19474- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TRIM21 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human kidney tissue. PVDF Membrane was probed with 0.5 µg/mL of Human TRIM21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6219) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TRIM21 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TRIM21 in Human PBMCs. TRIM21 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated for 8 hours with 20 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) using Human TRIM21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
