NB100-373

antibody from Novus Biologicals

Targeting: SMC2 CAP-E, hCAP-E, SMC2L1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-373

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-373, RRID:AB_2192488

Product name

Rabbit Polyclonal SMC2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Nesprin-2 Interacts with Condensin Component SMC2.

Xing X, Mroß C, Hao L, Munck M, Herzog A, Mohr C, Unnikannan CP, Kelkar P, Noegel AA, Eichinger L, Neumann S
International journal of cell biology 2017;2017:8607532

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PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression.

Liu W, Tanasa B, Tyurina OV, Zhou TY, Gassmann R, Liu WT, Ohgi KA, Benner C, Garcia-Bassets I, Aggarwal AK, Desai A, Dorrestein PC, Glass CK, Rosenfeld MG
Nature 2010 Jul 22;466(7305):508-12

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Quantitative nuclear proteomics identifies mTOR regulation of DNA damage response.

Bandhakavi S, Kim YM, Ro SH, Xie H, Onsongo G, Jun CB, Kim DH, Griffin TJ
Molecular & cellular proteomics : MCP 2010 Feb;9(2):403-14

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PICH and cotargeted Plk1 coordinately maintain prometaphase chromosome arm architecture.

Kurasawa Y, Yu-Lee LY
Molecular biology of the cell 2010 Apr 1;21(7):1188-99

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Differential regulation of telomere and centromere cohesion by the Scc3 homologues SA1 and SA2, respectively, in human cells.

Canudas S, Smith S
The Journal of cell biology 2009 Oct 19;187(2):165-73

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Identification of cellular proteins that interact with the adeno-associated virus rep protein.

Nash K, Chen W, Salganik M, Muzyczka N
Journal of virology 2009 Jan;83(1):454-69

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Condensin I recruitment and uneven chromatin condensation precede mitotic cell death in response to DNA damage.

Blank M, Lerenthal Y, Mittelman L, Shiloh Y
The Journal of cell biology 2006 Jul 17;174(2):195-206

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.