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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Flow cytometry [1]
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- Product number
- GTX81522 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81522, RRID:AB_11167650
- Product name
- PITX1 antibody, Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PITX1 Antibody (Center) (GTX81522) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PITX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of PITX1 antibody (Center) (GTX81522) in NCI-H460 cell line lysates (35ug/lane). PITX1 (arrow) was detected using the purified Pab.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of PITX1 (arrow) using rabbit polyclonal PITX1 Antibody (Center) (GTX81522). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PITX1 Antibody (Center) (GTX81522) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
