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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A302-672A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEK4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NEK4 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NEK4 (Cat. No. A302-672A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NEK4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody A302-672A used for IP at 3 µg/mg lysate. NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody A302-673A, which recognizes a downstream epitope. For blotting immunoprecipitated NEK4, A302-673A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
