A301-457A

antibody from Invitrogen Antibodies

Targeting: ZFC3H1 CCDC131, CSRC2, KIAA0546, MGC23401, PSRC2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-457A

Provider

Invitrogen Antibodies

Product name

CCDC131 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CCDC131 by western blot. Samples: Whole cell lysate (50 µg) from K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC131 antibody (Product # A301-457A; lot A301-457A-3) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CCDC131 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCDC131 antibody A301-457A (lot A301-457A- 2) used for IP at 6 µg per reaction. CCDC131 was also immunoprecipitated by a previous lot of this antibody (lot A301-457A-1) and rabbit anti-CCDC131 antibody A301-455A. For blotting immunoprecipitated CCDC131, A301-457A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CCDC131 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC131 antibody A301-457A (lot A301-457A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CCDC131 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCDC131 antibody (Product # A301-457A; lot A301-457A-3) used for IP at 6 µg per reaction. CCDC131 was also immunoprecipitated by a previous lot of this antibody (lot A301-457A-2). For blotting immunoprecipitated CCDC131, Product # A301-457A was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.