11880-1-AP

antibody from Proteintech Group

Targeting: KLF4 EZF, GKLF

Provider product page for 11880-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [69]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 11880-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11880-1-AP, RRID:AB_10640807
Product name: KLF4 antibody
Antibody type: Polyclonal
Description: KD/KO validated KLF4 antibody (Cat. #11880-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

KLF4 protein structure - 11880-1-AP shown in red.

Submitted references Toxicity evaluation of mesoporous silica particles Santa Barbara No. 15 amorphous in human umbilical vein endothelial cells: influence of particle morphology.

Teng W, Yang Z, Wang S, Xiong D, Chen Y, Wu Z
Journal of applied toxicology : JAT 2021 Sep;41(9):1467-1478

Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the MERTK locus.

Tang X, Fu J, Chen Z, Luo L, Li DW, Liu Y
Stem cell research 2021 May;53:102310

CDR1as regulated by hnRNPM maintains stemness of periodontal ligament stem cells via miR-7/KLF4.

Gu X, Li X, Jin Y, Zhang Z, Li M, Liu D, Wei F
Journal of cellular and molecular medicine 2021 May;25(9):4501-4515

Smooth muscle cell-specific knockout of interferon gamma (IFN-γ) receptor attenuates intimal hyperplasia via STAT1-KLF4 activation.

He L, Gao K, Liu H, Wang J, Li X, He C
Life sciences 2021 May 25;:119651

A novel Lnc408 maintains breast cancer stem cell stemness by recruiting SP3 to suppress CBY1 transcription and increasing nuclear β-catenin levels.

Wen S, Qin Y, Wang R, Yang L, Zeng H, Zhu P, Li Q, Qiu Y, Chen S, Liu Y, Hou Y, Tang X, Liu M, Tu G
Cell death & disease 2021 May 1;12(5):437

AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation.

Bian X, Shi D, Xing K, Zhou H, Lu L, Yu D, Wu W
Clinical and translational medicine 2021 Mar;11(3):e352

Effect of the interference with DRP1 expression on the biological characteristics of glioma stem cells.

Zhang L, Cao H, Tao H, Yang J, Gong W, Hu Q
Experimental and therapeutic medicine 2021 Jul;22(1):696

2-Methoxyestradiol synergizes with Erlotinib to suppress hepatocellular carcinoma by disrupting the PLAGL2-EGFR-HIF-1/2α signaling loop.

Zheng S, Ni J, Li Y, Lu M, Yao Y, Guo H, Jiao M, Jin T, Zhang H, Yuan A, Wang Z, Yang Y, Chen Z, Wu H, Hu W
Pharmacological research 2021 Jul;169:105685

Cellular reprogramming with multigene activation by the delivery of CRISPR/dCas9 ribonucleoproteins via magnetic peptide-imprinted chitosan nanoparticles.

Lee MH, Lin CC, Thomas JL, Li JA, Lin HY
Materials today. Bio 2021 Jan;9:100091

A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis.

Qin Y, Hou Y, Liu S, Zhu P, Wan X, Zhao M, Peng M, Zeng H, Li Q, Jin T, Cui X, Liu M
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021 Jan;8(2):2002232

Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats.

Zhang L, Tai Y, Tang S, Zhao C, Tong H, Gao J, Tang C
Digestive diseases and sciences 2021 Jan;66(1):131-142

The preventive and therapeutic effects of AAV1-KLF4-shRNA in cigarette smoke-induced pulmonary hypertension.

Sun D, Ding D, Li Q, Xie M, Xu Y, Liu X
Journal of cellular and molecular medicine 2021 Jan;25(2):1238-1251

Identification of an N6-methyladenosine-mediated positive feedback loop that promotes Epstein-Barr virus infection.

Dai DL, Li X, Wang L, Xie C, Jin Y, Zeng MS, Zuo Z, Xia TL
The Journal of biological chemistry 2021 Jan-Jun;296:100547

Cooperative activation of bovine herpesvirus 1 productive infection and viral regulatory promoters by androgen receptor and Krüppel-like transcription factors 4 and 15.

Sawant L, Thunuguntla P, Jones C
Virology 2021 Jan 2;552:63-72

BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation.

Yu S, Guo J, Sun Z, Lin C, Tao H, Zhang Q, Cui Y, Zuo H, Lin Y, Chen S, Liu H, Chen Z
Cell death & disease 2021 Feb 19;12(2):197

ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma.

Zhang M, Wang L, Yue Y, Zhang L, Liu T, Jing M, Liang X, Ma M, Xu S, Wang K, Wang X, Fan J
Journal of experimental & clinical cancer research : CR 2021 Feb 11;40(1):65

A Novel Van der Woude Syndrome-Causing IRF6 Variant Is Subject to Incomplete Non-sense-Mediated mRNA Decay Affecting the Phenotype of Keratinocytes.

Degen M, Girousi E, Feldmann J, Parisi L, La Scala GC, Schnyder I, Schaller A, Katsaros C
Frontiers in cell and developmental biology 2020;8:583115

Modulating cancer stemness provides luminal a breast cancer cells with HER2 positive-like features.

Mei Y, Cai D, Dai X
Journal of Cancer 2020;11(5):1162-1169

miR-182-3p/Myadm contribute to pulmonary artery hypertension vascular remodeling via a KLF4/p21-dependent mechanism.

Sun L, Lin P, Chen Y, Yu H, Ren S, Wang J, Zhao L, Du G
Theranostics 2020;10(12):5581-5599

The AKT/GSK3-Mediated Slug Expression Contributes to Oxaliplatin Resistance in Colorectal Cancer via Upregulation of ERCC1.

Wei W, Ma XD, Jiang GM, Shi B, Zhong W, Sun CL, Zhao L, Hou YJ, Wang H
Oncology research 2020 Sep 1;28(4):423-438

Evaluation of toxicity of halloysite nanotubes and multi-walled carbon nanotubes to endothelial cells in vitro and blood vessels in vivo.

Wu B, Jiang M, Liu X, Huang C, Gu Z, Cao Y
Nanotoxicology 2020 Oct;14(8):1017-1038

Pioneer transcription factors, progesterone receptor and Krüppel like transcription factor 4, cooperatively stimulate the bovine herpesvirus 1 ICP0 early promoter and productive late protein expression.

Sawant L, Wijesekera N, Jones C
Virus research 2020 Oct 15;288:198115

Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.

Lv Y, Wang X, Li X, Xu G, Bai Y, Wu J, Piao Y, Shi Y, Xiang R, Wang L
PLoS biology 2020 Nov;18(11):e3000872

Using inducible lentiviral vectors to generate induced pluripotent stem cell line ZOCi001-A from peripheral blood cells of a patient with CRB1^-/- retinitis pigmentosa.

Tang X, Liu X, Chen Z, Luo L, Liu X, Deng J, Li DW, Liu Y
Stem cell research 2020 May;45:101817

The rRNA m⁶A methyltransferase METTL5 is involved in pluripotency and developmental programs.

Ignatova VV, Stolz P, Kaiser S, Gustafsson TH, Lastres PR, Sanz-Moreno A, Cho YL, Amarie OV, Aguilar-Pimentel A, Klein-Rodewald T, Calzada-Wack J, Becker L, Marschall S, Kraiger M, Garrett L, Seisenberger C, Hölter SM, Borland K, Van De Logt E, Jansen PWTC, Baltissen MP, Valenta M, Vermeulen M, Wurst W, Gailus-Durner V, Fuchs H, Hrabe de Angelis M, Rando OJ, Kellner SM, Bultmann S, Schneider R
Genes & development 2020 May 1;34(9-10):715-729

Long Noncoding RNA HOX Transcript Antisense RNA Gene rs17720428 Single Nucleotide Polymorphism Is Associated with Gastric Cancer Risk and Prognosis.

Li Y, Zhang Z, Yang Y, Ma J
Genetic testing and molecular biomarkers 2020 Jan;24(1):38-46

Deoxycholic Acid Upregulates the Reprogramming Factors KFL4 and OCT4 Through the IL-6/STAT3 Pathway in Esophageal Adenocarcinoma Cells.

Chen M, Ye A, Wei J, Wang R, Poon K
Technology in cancer research & treatment 2020 Jan-Dec;19:1533033820945302

Oxidative stress-induced KLF4 activates inflammatory response through IL17RA and its downstream targets in retinal pigment epithelial cells.

Sun Q, Gong L, Qi R, Qing W, Zou M, Ke Q, Zhang L, Tang X, Nie Q, Yang Y, Hu A, Ding X, Lu L, Liu Y, Li DW
Free radical biology & medicine 2020 Feb 1;147:271-281

Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the CRB1 locus.

Tang X, Chen Z, Tan X, Luo L, Liu X, Gong L, Li DW, Liu Y
Stem cell research 2020 Dec;49:102057

Upregulation of the microRNA rno-miR-146b-5p may be involved in the development of intestinal injury through inhibition of Kruppel-like factor 4 in intestinal sepsis.

Tong L, Tang C, Cai C, Guan X
Bioengineered 2020 Dec;11(1):1334-1349

Multi-walled carbon nanotubes decrease neuronal NO synthase in 3D brain organoids.

Jiang Y, Gong H, Jiang S, She C, Cao Y
The Science of the total environment 2020 Dec 15;748:141384

miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis.

Shi DM, Shi XL, Xing KL, Zhou HX, Lu LL, Wu WZ
Cellular signalling 2020 Aug;72:109650

KLF4-mediated upregulation of CD9 and CD81 suppresses hepatocellular carcinoma development via JNK signaling.

Li Y, Yu S, Li L, Chen J, Quan M, Li Q, Gao Y
Cell death & disease 2020 Apr 29;11(4):299

KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling.

Li L, Yu S, Wu Q, Dou N, Li Y, Gao Y
International journal of biological sciences 2019;15(5):953-961

miR-92b-3p Functions As A Key Gene In Esophageal Squamous Cell Cancer As Determined By Co-Expression Analysis.

Wang W, Fu S, Lin X, Zheng J, Pu J, Gu Y, Deng W, Liu Y, He Z, Liang W, Wang C
OncoTargets and therapy 2019;12:8339-8353

SIRT1 activation alleviates brain microvascular endothelial dysfunction in peroxisomal disorders.

Zhang Y, Cui G, Wang Y, Gong Y, Wang Y
International journal of molecular medicine 2019 Sep;44(3):995-1005

SP1 regulates KLF4 via SP1 binding motif governed by DNA methylation during odontoblastic differentiation of human dental pulp cells.

Sun Z, Yu S, Chen S, Liu H, Chen Z
Journal of cellular biochemistry 2019 Sep;120(9):14688-14699

Multi-walled carbon nanotubes promoted lipid accumulation in human aortic smooth muscle cells.

Yang H, Li J, Yang C, Liu H, Cao Y
Toxicology and applied pharmacology 2019 Jul 1;374:11-19

LncRNA NKILA regulates endothelium inflammation by controlling a NF-κB/KLF4 positive feedback loop.

Zhu X, Du J, Yu J, Guo R, Feng Y, Qiao L, Xu Z, Yang F, Zhong G, Liu F, Cheng F, Chu M, Lin J
Journal of molecular and cellular cardiology 2019 Jan;126:60-69

Klf4 Promotes Dentinogenesis and Odontoblastic Differentiation via Modulation of TGF-β Signaling Pathway and Interaction With Histone Acetylation.

Tao H, Lin H, Sun Z, Pei F, Zhang J, Chen S, Liu H, Chen Z
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2019 Aug;34(8):1502-1516

Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6.

Li Z, Jia Y, Han S, Wang X, Han F, Zhang J, Zhang W, Guan H, Hu D
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;47(6):2278-2290

SRC-3 protects intestine from DSS-induced colitis by inhibiting inflammation and promoting goblet cell differentiation through enhancement of KLF4 expression.

Chen W, Zhuo M, Lu X, Xia X, Zhao Y, Huang Z, Xu J, Li W, Yu C
International journal of biological sciences 2018;14(14):2051-2064

Role of Krüppel-like factor 4 in cigarette smoke-induced pulmonary vascular remodeling.

Sun D, Li Q, Ding D, Li X, Xie M, Xu Y, Liu X
American journal of translational research 2018;10(2):581-591

Quaking promotes the odontoblastic differentiation of human dental pulp stem cells.

Li S, Lin C, Zhang J, Tao H, Liu H, Yuan G, Chen Z
Journal of cellular physiology 2018 Sep;233(9):7292-7304

Disruption of OCT4 Ubiquitination Increases OCT4 Protein Stability and ASH2L-B-Mediated H3K4 Methylation Promoting Pluripotency Acquisition.

Li S, Xiao F, Zhang J, Sun X, Wang H, Zeng Y, Hu J, Tang F, Gu J, Zhao Y, Jin Y, Liao B
Stem cell reports 2018 Oct 9;11(4):973-987

Generation of an iPS cell line via a non-integrative method using urine-derived cells from a patient with USH2A-associated retinitis pigmentosa.

Guo Y, Zeng Q, Liu S, Yu Q, Wang P, Ma H, Shi S, Yan X, Cui Z, Xie M, Xue Y, Zha Q, Li Z, Zhang J, Tang S, Chen J
Stem cell research 2018 May;29:139-142

Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.

Ye B, Liu B, Hao L, Zhu X, Yang L, Wang S, Xia P, Du Y, Meng S, Huang G, Qin X, Wang Y, Yan X, Li C, Hao J, Zhu P, He L, Tian Y, Fan Z
Nature communications 2018 Mar 28;9(1):1261

SIRT1 deacetylates KLF4 to activate Claudin-5 transcription in ovarian cancer cells.

Zhang X, Chen J, Sun L, Xu Y
Journal of cellular biochemistry 2018 Feb;119(2):2418-2426

The histone methyltransferase SETD1A regulates thrombomodulin transcription in vascular endothelial cells.

Li Z, Chen B, Weng X, Yu L, Song M, Fang M, Guo J, Xu Y
Biochimica et biophysica acta. Gene regulatory mechanisms 2018 Aug;1861(8):752-761

TGFβ1 Promotes Gemcitabine Resistance through Regulating the LncRNA-LET/NF90/miR-145 Signaling Axis in Bladder Cancer.

Zhuang J, Shen L, Yang L, Huang X, Lu Q, Cui Y, Zheng X, Zhao X, Zhang D, Huang R, Guo H, Yan J
Theranostics 2017;7(12):3053-3067

Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells.

Liu GD, Zhou SF, Ding XC, Fang CL, Mi SY, Gao XC, Han Q
International journal of ophthalmology 2017;10(4):560-566

MicroRNA-145 protects follicular granulosa cells against oxidative stress-induced apoptosis by targeting Krüppel-like factor 4.

Xu L, Sun H, Zhang M, Jiang Y, Zhang C, Zhou J, Ding L, Hu Y, Yan G
Molecular and cellular endocrinology 2017 Sep 5;452:138-147

CHD1L Promotes Neuronal Differentiation in Human Embryonic Stem Cells by Upregulating PAX6.

Dou D, Zhao H, Li Z, Xu L, Xiong X, Wu X, Sun Y, Zeng S, Ouyang Q, Zhou D, Ma N, Lin G, Hu L
Stem cells and development 2017 Nov 15;26(22):1626-1636

MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells.

Minami K, Taniguchi K, Sugito N, Kuranaga Y, Inamoto T, Takahara K, Takai T, Yoshikawa Y, Kiyama S, Akao Y, Azuma H
Oncotarget 2017 May 16;8(20):33064-33077

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Wang L, Zhang T, Wang L, Cai Y, Zhong X, He X, Hu L, Tian S, Wu M, Hui L, Zhang H, Gao P
The EMBO journal 2017 May 15;36(10):1330-1347

Notch signaling plays a crucial role in cancer stem-like cells maintaining stemness and mediating chemotaxis in renal cell carcinoma.

Xiao W, Gao Z, Duan Y, Yuan W, Ke Y
Journal of experimental & clinical cancer research : CR 2017 Mar 9;36(1):41

microRNA let-7g suppresses PDGF-induced conversion of vascular smooth muscle cell into the synthetic phenotype.

Wang TM, Chen KC, Hsu PY, Lin HF, Wang YS, Chen CY, Liao YC, Juo SH
Journal of cellular and molecular medicine 2017 Dec;21(12):3592-3601

miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4.

Zhai F, Cao C, Zhang L, Zhang J
Oncotarget 2017 Aug 29;8(35):59246-59256

Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury.

Zhou B, Zeng S, Li N, Yu L, Yang G, Yang Y, Zhang X, Fang M, Xia J, Xu Y
Arteriosclerosis, thrombosis, and vascular biology 2017 Apr;37(4):675-684

miR-103 Promotes Proliferation and Metastasis by Targeting KLF4 in Gastric Cancer.

Zheng J, Liu Y, Qiao Y, Zhang L, Lu S
International journal of molecular sciences 2017 Apr 26;18(5)

Aldehyde dehydrogenase 1A1 up-regulates stem cell markers in benzo[a]pyrene-induced malignant transformation of BEAS-2B cells.

Liu Y, Lu R, Gu J, Chen Y, Zhang X, Zhang L, Wu H, Hua W, Zeng J
Environmental toxicology and pharmacology 2016 Jul;45:241-50

LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.

Zhu P, Wang Y, Wu J, Huang G, Liu B, Ye B, Du Y, Gao G, Tian Y, He L, Fan Z
Nature communications 2016 Dec 1;7:13608

Vacuolar ATPase in phagosome-lysosome fusion.

Kissing S, Hermsen C, Repnik U, Nesset CK, von Bargen K, Griffiths G, Ichihara A, Lee BS, Schwake M, De Brabander J, Haas A, Saftig P
The Journal of biological chemistry 2015 May 29;290(22):14166-80

Lithium chloride promotes the odontoblast differentiation of hair follicle neural crest cells by activating Wnt/β-catenin signaling.

Shan T, Zhou C, Yang R, Yan F, Zhang P, Fu Y, Jiang H
Cell biology international 2015 Jan;39(1):35-43

Krüppel-like factor 4 synergizes with CREB to increase the activity of apolipoprotein E gene promoter in macrophages.

Stavri S, Simionescu M, Kardassis D, Gafencu AV
Biochemical and biophysical research communications 2015 Dec 4-11;468(1-2):66-72

The Janus-faced roles of Krüppel-like factor 4 in oral squamous cell carcinoma cells.

Li W, Liu M, Su Y, Zhou X, Liu Y, Zhang X
Oncotarget 2015 Dec 29;6(42):44480-94

Photodynamic therapy-mediated cancer vaccination enhances stem-like phenotype and immune escape, which can be blocked by thrombospondin-1 signaling through CD47 receptor protein.

Zheng Y, Zou F, Wang J, Yin G, Le V, Fei Z, Liu J
The Journal of biological chemistry 2015 Apr 3;290(14):8975-86

LSD1 regulates pluripotency of embryonic stem/carcinoma cells through histone deacetylase 1-mediated deacetylation of histone H4 at lysine 16.

Yin F, Lan R, Zhang X, Zhu L, Chen F, Xu Z, Liu Y, Ye T, Sun H, Lu F, Zhang H
Molecular and cellular biology 2014 Jan;34(2):158-79

Pluripotent stem cell protein Sox2 confers sensitivity to LSD1 inhibition in cancer cells.

Zhang X, Lu F, Wang J, Yin F, Xu Z, Qi D, Wu X, Cao Y, Liang W, Liu Y, Sun H, Ye T, Zhang H
Cell reports 2013 Oct 31;5(2):445-57

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 11880-1-AP(KLF4 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Confocal immunofluorescent analysis of human embronic stem cells with 11880-1-AP at dilution of 1:50. The PE shows staining with 11880-1-AP/PE. The DAPI shows nuclear staining by DAPI.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-KLF4 (IP:11880-1-AP, 3ug; Detection:11880-1-AP 1:500) with HeLa cells lysate 4650ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon using 11880-1-AP(KLF4 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue