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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- AM2725A - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM2725a, RRID:AB_1278084
- Product name
- KLF4 Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Purified Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.46 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references KLF4α up-regulation promotes cell cycle progression and reduces survival time of patients with pancreatic cancer.
Wei D, Wang L, Kanai M, Jia Z, Le X, Li Q, Wang H, Xie K
Gastroenterology 2010 Dec;139(6):2135-45
Gastroenterology 2010 Dec;139(6):2135-45
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of lysate from KLF4 protein, using KLF4 Antibody(Cat. #AM2725a). AM2725a was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Immunoflurescence analysis of KLF4 antibody , AM2725a (5 ug/ml). Hela cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection."
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
