- GTX101508 - GeneTex KLF4 antibody

Submitted references The antipsychotic chlorpromazine suppresses YAP signaling, stemness properties, and drug resistance in breast cancer cells.

Yang CE, Lee WY, Cheng HW, Chung CH, Mi FL, Lin CW
Chemico-biological interactions 2019 Apr 1;302:28-35

Generation and characterization of a human iPSC line from an ALS patient carrying the Q66K-MATR3 mutation.

Pollini D, Loffredo R, Cardano M, Conti L, Lattante S, Notarangelo A, Sabatelli M, Provenzani A
Stem cell research 2018 Dec;33:146-150

K48-linked KLF4 ubiquitination by E3 ligase Mule controls T-cell proliferation and cell cycle progression.

Hao Z, Sheng Y, Duncan GS, Li WY, Dominguez C, Sylvester J, Su YW, Lin GH, Snow BE, Brenner D, You-Ten A, Haight J, Inoue S, Wakeham A, Elford A, Hamilton S, Liang Y, Zúñiga-Pflücker JC, He HH, Ohashi PS, Mak TW
Nature communications 2017 Jan 13;8:14003

Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.

Lin HC, Lin MH, Liao JH, Wu TH, Lee TH, Mi FL, Wu CH, Chen KC, Cheng CH, Lin CW
Journal of agricultural and food chemistry 2017 Jan 11;65(1):51-59

Arsenite inhibits the function of CD133⁺ CD13⁺ liver cancer stem cells by reducing PML and Oct4 protein expression.

Tang H, Jin Y, Jin S, Tan Z, Peng Z, Kuang Y
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Oct;37(10):14103-14115

DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma.

Li HK, Mai RT, Huang HD, Chou CH, Chang YA, Chang YW, You LR, Chen CM, Lee YH
Scientific reports 2016 Jun 27;6:28637

Down-regulation of the oncogene PTTG1 via the KLF6 tumor suppressor during induction of myeloid differentiation.

Chen PY, Yen JH, Kao RH, Chen JH
PloS one 2013;8(8):e71282

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE GTX101508 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (20 ?g of whole cell lysate) A: human ESC 10% SDS PAGE GTX101508 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX101508 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (20 ?g of whole cell lysate) A: mouse ESC 12% SDS PAGE GTX101508 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HCT116 whole cell and nuclear extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 £gg of KLF4 antibody (GTX101508).Western blot analysis was performed using KLF4 antibody (GTX101508).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Flow cytometry on human embryonic stem cells, staining with KLF4 (GTX101508)antibody at 1:100 dilution(purple) or rabbit IgG (black).

GTX101508

Antibody data