PA5-27441

antibody from Invitrogen Antibodies

Targeting: KLF4 EZF, GKLF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27441

Provider

Invitrogen Antibodies

Product name

KLF4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa.

Concentration

1.0 mg/mL

References

Submitted references

Inactivation of Cerebral Cavernous Malformation Genes Results in Accumulation of von Willebrand Factor and Redistribution of Weibel-Palade Bodies in Endothelial Cells.

Much CD, Sendtner BS, Schwefel K, Freund E, Bekeschus S, Otto O, Pagenstecher A, Felbor U, Rath M, Spiegler S
Frontiers in molecular biosciences 2021;8:622547

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Precise CCM1 gene correction and inactivation in patient-derived endothelial cells: Modeling Knudson's two-hit hypothesis in vitro.

Spiegler S, Rath M, Much CD, Sendtner BS, Felbor U
Molecular genetics & genomic medicine 2019 Jul;7(7):e00755

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KLF4 using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a KLF4 polyclonal antibody (Product # PA5-27441) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of KLF4 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a KLF4 Polyclonal Antibody (Product # PA5-27441) at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

KLF4 Polyclonal Antibody detects KLF4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: KLF4 protein stained by KLF4 Polyclonal Antibody (Product # PA5-27441) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

KLF4 Polyclonal Antibody detects KLF4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: KLF4 protein stained by KLF4 Polyclonal Antibody (Product # PA5-27441) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

KLF4 Polyclonal Antibody detects KLF4 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KLF4 Polyclonal Antibody (Product # PA5-27441) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

KLF4 Polyclonal Antibody detects KLF4 protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. KLF4 Polyclonal Antibody (Product # PA5-27441) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 2 Characterization of CCM1 +/- and clonally expanded CCM1 -/- BOECS. (A) CCM1 -/- BOECs were established by limiting dilution of the CRISPR/Cas9 RNP-treated cell pool and clonal expansion of single cells (created with BioRender.com). (B) Shown are the genotypes of CCM1 -/- BOEC clones included in this study. All variants either lead to a frameshift or a premature stop codon (additional information on the CRISPR/Cas9-induced mutations and numbers of individual clones are given in Supplementary Table S1 ). (C) CCM1 -/- BOECs presented a more compact morphology in brightfield microscopy and increased tube formation. The largest cell diameter and the number of meshes formed on Matrigel-coated plates were quantified. Scale bar indicates 400 um. Data are presented as single data points with the mean ( n = 4-6). (D) Immunofluorescence staining revealed a higher number of intercellular gaps (white arrowheads), more actin stress fibers, and a higher expression of KLF2 and KLF4 in CCM1 -/- BOECs. Representative images are shown. Scale bar indicates 100 um. Individual data points are shown with the mean ( n = 3-5). Student's t test was used for statistical analysis: * p < 0.05; ** p < 0.01; **** p < 0.0001; ns = not significant; VE-cadherin = vascular endothelial cadherin.